22 264

Cited 31 times in

Transactivation of the mouse sulfonylurea receptor I gene by BETA2/NeuroD.

Authors
Kim, JW; Seghers, V; Cho, JH; Kang, Y; Kim, S; Ryu, Y; Baek, K; Aguilar-Bryan, L; Lee, YD; Bryan, J; Suh-Kim, H
Citation
Molecular endocrinology (Baltimore, Md.), 16(5):1097-1107, 2002
Journal Title
Molecular endocrinology (Baltimore, Md.)
ISSN
0888-88091944-9917
Abstract
The sulfonylurea receptor 1 (SUR1) plays a key role in regulation of insulin secretion in pancreatic beta-cells. In this study we investigated the mechanism for tissue-specific expression of the SUR1 gene. A -138/-20 fragment exhibited basal promoter activity while the -660/-20 fragment contained a regulatory element for tissue-specific expression of the mouse SUR1 gene. A pancreatic beta-cell-specific transcription factor, BETA2 (beta-cell E box transcription factor)/NeuroD, enhanced the promoter activity of the -660/-20 fragment in cooperation with E47. Coexpression of a dominant negative mutant of BETA2/NeuroD, BETA2(1-233), repressed the promoter activity of the -660/-20 fragment. BETA2/NeuroD bound specifically to the E3 element located at -141. The E3 sequence in a heterologous context conferred transactivation by BETA2/NeuroD in HeLa and HIT cells. Mutation of E3 eliminated the stimulatory effect of BETA2/NeuroD. Unlike BETA2/NeuroD, neurogenin 3 (ngn3) could not activate the E3 element in HeLa cells. Overexpression of ngn3 concomitantly increased expression of BETA2/NeuroD and SUR1 in HIT cells but not in HeLa cells. These results indicate that BETA2/NeuroD induces tissue-specific expression of the SUR1 gene through the E3 element. These results also suggest that E3 is specific for BETA2/NeuroD, and the stimulatory effect of ngn3 in HIT cells may require factors specifically expressed in HIT cells.
MeSH terms
*ATP-Binding Cassette TransportersAnimalsBase SequenceBasic Helix-Loop-Helix Transcription FactorsBinding SitesDNA-Binding Proteins/metabolism/*pharmacologyGene ExpressionHela CellsHumansInsulinomaMiceMolecular Sequence DataMutagenesisPancreatic NeoplasmsPeptide Fragments/genetics/metabolismPotassium Channels/*genetics/metabolism*Potassium Channels, Inwardly RectifyingPromoter Regions, GeneticRatsReceptors, Drug/*genetics/metabolismRegulatory Sequences, Nucleic AcidReverse Transcriptase Polymerase Chain ReactionTrans-Activators/metabolism/*pharmacology*Transcriptional ActivationTransfectionTumor Cells, Cultured
DOI
10.1210/mend.16.5.0934
PMID
11981044
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
강, 엽이, 영돈서, 해영
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse