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Localization of inducible nitric oxide synthase and endothelial constitutive nitric oxide synthase in airway mucosa of toluene diisocyanate-induced asthma.

Authors
Lee, SK; Park, HS; Lim, HE; Kim, SS; Nahm, DH; Lee, YM; Park, CS
Citation
Allergy and asthma proceedings, 24(4):275-280, 2003
Journal Title
Allergy and asthma proceedings
ISSN
1088-54121539-6304
Abstract
Endogenous exhaled nitric oxide (NO) is increased in asthma patients, especially in the exacerbated state. To evaluate the role of NO in airway mucosa of toluene diisocyanate (TDI)-induced asthma in relation to clinical and immunologic findings, 20 TDI-induced asthma patients were enrolled and classified into two groups: 9 newly diagnosed patients (group I) and 11 patients having persistent asthma symptoms for > 5 years despite work withdrawal (group II). Immunohistocytochemistry was applied to compare the expression of endothelial constitutive NOS (c-NOS) and induced NOS (i-NOS) in airway mucosa of both groups. They were observed in four areas: epithelium (EP), smooth muscle (SM), vascular endothelium (VE), and mucous gland (MG). The intensity of expression was graded on a scale of one to four and mean values were presented from blind readings observed by two experts. Serum-specific immunoglobulin E (IgE) and immunoglobulin G (IgG) antibodies to TDI-human serum albumin conjugate were measured by enzyme-linked immunosorbent assay. e-NOS was found most frequently in VE (5/6 [83.3] versus 7/10 [70%]) followed by MG, EP, and SM in both groups, i-NOS was found most frequently in EP (7/9 [77.8%] versus 7/11 [63.6%]), followed by MG, VE, and SM in both groups. The intensities of i-NOS and c-NOS of MG were significantly higher in group I than in group II (p 0.05, respectively). There were no significant correlations between i-NOS or c-NOS expression and exposure or asthma symptom duration (p > 0.05). No associations were found between i-NOS or c-NOS expression and the presence of specific IgE or IgG to TDI-human serum albumin conjugate (p > 0.05, respectively). In conclusion, i-NOS and c-NOS expressions were noted in EP, SM, VE, and MG in airway mucosa of TDI-induced asthma patients, which may contribute to airway inflammation.
MeSH terms
AdultAsthma/chemically inducedAsthma/enzymology*FemaleHumansImmunoglobulin E/bloodImmunoglobulin G/bloodImmunohistochemistryMaleNitric Oxide/physiology*Nitric Oxide Synthase/analysis*Nitric Oxide Synthase Type IINitric Oxide Synthase Type IIIRespiratory Mucosa/enzymology*Toluene 2,4-Diisocyanate
PMID
12974195
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
AJOU Authors
박, 해심임, 현이남, 동호
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