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Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.

Authors
Lee, YM; Kim, W
Citation
The Biochemical journal, 374(Pt 2):497-503, 2003
Journal Title
The Biochemical journal
ISSN
0264-60211470-8728
Abstract
A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.
MeSH terms
Amino Acid SequenceBRCA2 Protein/metabolism*DNA-Binding Proteins/chemistryDNA-Binding Proteins/metabolism*Gene LibraryGenetic Vectors/geneticsHela CellsHumansKinesin/chemistryKinesin/metabolism*Leucine ZippersMolecular Sequence DataNeoplasm Proteins/geneticsPeptides/metabolismProtein BindingProtein Interaction Mapping/methods*Protein Structure, TertiarySaccharomyces cerevisiae/geneticsTumor Cells, CulturedTwo-Hybrid System Techniques
DOI
10.1042/BJ20030452
PMID
12809554
Appears in Collections:
Journal Papers > Research Organization > Institute for Medical Sciences
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
이, 영미김, 완기
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