1 207

Cited 69 times in

Reversion from precore/core promoter mutants to wild-type hepatitis B virus during the course of lamivudine therapy.

Authors
Cho, SW; Hahm, KB; Kim, JH
Citation
Hepatology (Baltimore, Md.), 32(5):1163-1169, 2000
Journal Title
Hepatology (Baltimore, Md.)
ISSN
0270-91391527-3350
Abstract
The effect of lamivudine administration on the evolution of precore/core promoter mutation is unknown. The aim of this study was to determine the changes of precore/core promoter sequences in chronic type B hepatitis patients treated with lamivudine. Serial sera were obtained from 11 patients before, at the beginning of, and during therapy. Serum samples were polymerase chain reaction-amplified, and nucleotide sequences of hepatitis B virus (HBV) were analyzed. At baseline, precore and core promoter mutations were found in 6 and 4 of 11 patients, respectively. A precore stop codon mutant was replaced by a wild-type virus in all 6 patients infected with precore mutant at a median treatment of 12 months (vs. before therapy; P =.011). Mutations in the core promoter appeared in only 1 of 10 patients (vs. before therapy; P =.021). However, precore and core promoter mutations appeared in 5 and 7 of 10 patients at a median treatment of 21 months, respectively. Acute exacerbation occurred after lamivudine withdrawal in 2 patients who had hepatitis B e antigen (HBeAg) loss or seroconversion. The serum remained HBeAg-negative throughout the study period, and each of 2 patients had precore wild-type virus during acute exacerbation. HBV mutants with core gene deletions are not eliminated completely during prolonged therapy in 2 patients in whom the HBV genomes had core gene deletions at baseline. In conclusion, lamivudine therapy resulted in reversion from precore/core promoter mutants to wild-type. However, mutations in the precore and core promoter region reappeared during prolonged therapy. HBeAg-negative wild-type precore hepatitis B virus could be selected after lamivudine withdrawal in patients who had HBeAg loss or seroconversion.
MeSH terms
AdultFemaleGene DeletionHepatitis B e Antigens/analysisHepatitis B virus/*drug effects/*geneticsHepatitis B, Chronic/drug therapy/immunology/virologyHumansLamivudine/*therapeutic useMaleMiddle AgedMutation/*drug effectsPromoter Regions, Genetic/*geneticsReverse Transcriptase Inhibitors/*therapeutic useViral Core Proteins/*genetics
DOI
10.1053/jhep.2000.19618
PMID
11050070
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
AJOU Authors
조, 성원함, 기백김, 진홍
Files in This Item:
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse