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Production and characterization of immortalized rat hepatocytes secreting hepatocyte growth factor/scatter factor.

Authors
Lee, JH; Kim, WH; Park, H; Yun, C; Kim, BH; Kwak, SJ; Cho, H; Kim, MW
Citation
Hepato-gastroenterology, 47(34):978-983, 2000
Journal Title
Hepato-gastroenterology
ISSN
0172-6390
Abstract
BACKGROUND/AIMS: Hepatocyte transplantation is a recently attractive field in the treatment of liver failure and enzyme-deficient diseases. However, procurement of sufficient quantities of hepatocytes is almost impossible. We attempted to create a hepatocyte cell line that could be used for hepatocyte transplantation.



METHODOLOGY: L2A2 is a conditionally immortalized rat hepatocyte cell line produced by transfection of temperature-sensitive simian virus T antigen to the hepatocytes in the Lewis rat. Hepatocyte Growth Factor/Scatter Factor (HGF/SF)-secreting L2A2 cells, designated as SF-21, was produced by transfecting human HGF/SF cDNA into L2A2 cells.



RESULTS: This cell line was able to produce HGF/SF at the rate of 5-10 ng/10(6) cells/24 hrs, and the recombinant HGF/SF was of the expected size and was functionally active in that it could scatter Madin-Darby canine kidney cells. The SF-21 cells grew faster than its parental cell clone, and survived and proliferated at 37 degrees C in vitro. Also, the SF-21 cells were able to survive and proliferate when transplanted into the spleen of syngeneic rat, and expressed glucose-6-phosphatase.



CONCLUSIONS: These HGF/SF-secreting hepatocytes can be used as a model system to test a feasibility of using genetically engineered hepatocyte cell line for hepatocyte transplantation in the rat.
MeSH terms
AnimalsBlotting, WesternCell Culture Techniques/*methodsCell LineHepatocyte Growth Factor/*secretionHepatocytes/*secretion/transplantationImmunohistochemistryMaleRatsRats, Inbred Lew/geneticsTransfection
PMID
11020861
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
AJOU Authors
이, 재호김, 욱환조, 혜성김, 명욱
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