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Selective enhancement of non-NMDA receptor-mediated responses following induction of long-term potentiation in entorhinal cortex.

Authors
Yun, SH; huh, K; Jung, MW
Citation
Synapse (New York, N.Y.), 35(1):1-7, 2000
Journal Title
Synapse (New York, N.Y.)
ISSN
0887-44761098-2396
Abstract
The contribution of NMDA receptors to the expression of long-term potentiation (LTP) is controversial. In entorhinal cortex (EC) previous studies reported either that LTP was exclusively expressed through NMDA receptors or that both NMDA and non-NMDA receptors were involved in LTP expression. To reexamine this issue, horizontal entorhinal cortical slices were prepared from adult rats and electrical stimulation was delivered in layer II/III, while field potential recordings were made in layer III. In the standard condition (2.5 mM Mg(++)), LTP was reliably induced by theta burst stimulation, but was blocked by 100 microM D-AP5, an NMDA receptor antagonist. This corroborates previous reports that NMDA receptor activation is required for induction of EC LTP. The field potential response was not affected by D-AP5, but completely blocked by 10 microM CNQX, a non-NMDA receptor antagonist. This indicates that the expression of LTP is mediated by non-NMDA receptors in the standard condition. LTP of NMDA receptor-mediated responses was tested by comparing NMDA responses before and after applying theta burst stimulation in medium containing low magnesium (0.4-1 mM). Theta burst stimulation induced 43.2+/-9.7% increase of non-NMDA responses (i.e., AP5-insensitive fast component) but 5.6+/-9.0% decrease of the NMDA receptor component (AP5-sensitive slow component). These results indicate that activation of NMDA receptors is critical for induction of LTP, but LTP expression is mediated by non-NMDA receptors in EC under these experimental conditions.
MeSH terms
2-Amino-5-phosphonovalerate/pharmacology6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacologyAnimalsEntorhinal Cortex/*physiologyExcitatory Amino Acid Antagonists/pharmacologyLong-Term Potentiation/*physiologyMaleRatsRats, Sprague-DawleyReceptors, N-Methyl-D-Aspartate/*physiologySynaptic Transmission/physiology
DOI
10.1002/(SICI)1098-2396(200001)35:1<1::AID-SYN1>3.0.CO;2-L
PMID
10579802
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
Journal Papers > Research Organization > Institute for Medical Sciences
AJOU Authors
허, 균정, 민환
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