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Postnatal changes in the calcium binding proteins of mouse testis.

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dc.contributor.authorGye, MC-
dc.contributor.authorKim, C-
dc.contributor.authorAhn, HS-
dc.contributor.authorKim, YS-
dc.date.accessioned2011-08-23T01:58:43Z-
dc.date.available2011-08-23T01:58:43Z-
dc.date.issued2001-
dc.identifier.issn0148-5016-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3890-
dc.description.abstractTo define the changes in calcium-binding proteins (CBPs) of mouse testis during postnatal development, testicular protein extracts were analyzed by mobility shift after Ca2+ binding (MSACB) in the 2-dimensional SDS-PAGE. In the adult testis extracts, 29 kinds of protein spots showed mobility shift in the gel containing Ca2+. Among them, 2 spots showed nonspecific mobility shift and 4 spots showed Zn2+ shift. Drastic changes in expression of high-Mr CBPs (Mr > 25 kDa) were observed in testis 3 weeks after birth, suggesting that their expressions were developmentally changed. There was no obvious difference in the qualitative patterns of CBP between 4-week-old testis and adult testis. There was a large mass of CBPs (Mr = 20-25 kDa) expressed constitutively in testis throughout the postnatal development. Degree of MSACB (Mr on the diagonal line/Mr of shifted point in Ca2+ gel) of each spot was in the range of 1.1-1.7 in 12% acrylamide gel. These results suggest that changes in CBPs of testis are closely related with progression of spermatogenesis in mouse testis.en
dc.formatapplication/pdf-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHCalcium-Binding Proteins-
dc.subject.MESHElectrophoresis, Polyacrylamide Gel-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred ICR-
dc.subject.MESHTestis-
dc.titlePostnatal changes in the calcium binding proteins of mouse testis.-
dc.typeArticle-
dc.identifier.pmid11204618-
dc.contributor.affiliatedAuthor안, 현수-
dc.contributor.affiliatedAuthor김, 영수-
dc.type.localJournal Papers-
dc.identifier.doi10.1080/01485010150211155-
dc.citation.titleArchives of andrology-
dc.citation.volume46-
dc.citation.number1-
dc.citation.date2001-
dc.citation.startPage51-
dc.citation.endPage55-
dc.identifier.bibliographicCitationArchives of andrology, 46(1). : 51-55, 2001-
dc.identifier.eissn1521-0375-
dc.relation.journalidJ001485016-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Urology
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