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Activation of mouse B lymphocyte by proteins containing hexahistidine.

Authors
Kim, KH; Kim, KS; Choi, SE; Kim, JK; Park, JH; Lee, JH; Kang, Y
Citation
Molecules and cells, 11(3):287-294, 2001
Journal Title
Molecules and cells
ISSN
1016-84780219-1032
Abstract
Many recombinant proteins are produced as a fusion protein tagging hexahistidine, which has been used for studying their biological function both in vitro and in vivo. Unexpectedly, we observed activation of BALB/c mouse splenocytes when treated with hexahistidine-tagged recombinant proteins. This activation was hexahistidine-specific since the anti-pentahistidine antibody completely neutralized the effect, and the bovine serum albumin conjugated with the hexahistidine peptides also showed a similar activation effect. The B cells seemed to be the activated splenocytes, since the cell population stained with the anti-immunoglobulin antibody and anti-CD80 antibody was increased after the treatment. However, the activation signal by hexahistidine was insufficient to fully differentiate the B lymphocytes. This result suggests that caution must be taken in the use of hexahistidine-tagged recombinant proteins, due to their nonspecific activation of B lymphocyte.
MeSH terms
AnimalsB-Lymphocytes/cytologyB-Lymphocytes/immunology*Cell Differentiation/immunologyCell Division/immunologyFemaleFlow CytometryHistidine*Lymphocyte Activation/immunology*MiceMice, Inbred BALB CRecombinant Fusion Proteins/immunology*Spleen/cytologyViral Envelope Proteins/immunology
PMID
11459217
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
강, 엽
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