8 275

Cited 0 times in

Activation of mouse macrophage by soluble endogenous murine leukemia virus (MuLV) envelope protein.

Authors
Lee, SE; Choi, SE; Kim, JH; Kim, KS; Kang, Y
Citation
Immunology letters, 79(3):221-227, 2001
Journal Title
Immunology letters
ISSN
0165-24781879-0542
Abstract
We identified recently an endogenous murine leukemia virus (MuLV) envelope protein as a new autoantigen reactive with autoimmune diabetic mouse sera and observed immunosuppressive activity of this envelope protein. In the present study, to elucidate the mechanism involved, we treated macrophages with the envelope protein and investigated activation of macrophage. We found enhancements of iNOS mRNA and nitrite in envelope protein-treated RAW264.7 cells and peritoneal macrophages. The stimulation was highly envelope protein-specific, and also time- and dose-dependent. The activation pattern was similar to that elicited by lipopolysaccharide (LPS) since the envelope protein showed a synergistic effect on macrophage activation in conjunction with interferon gamma (IFN-gamma). Furthermore, deacylated LPS as a competitive inhibitor of LPS showed inhibition of envelope protein-mediated macrophage activation. These data show that MuLV envelope protein can be a new macrophage activator and suggest that the retroviral envelope protein may elicit immunosuppressive activity through macrophage activation.
MeSH terms
AnimalsCell LineCell SurvivalCells, CulturedHot TemperatureInterferon-gamma/pharmacologyLeukemia Virus, Murine/immunology*Lipopolysaccharides/pharmacologyMacrophage Activation*/drug effectsMacrophages/drug effectsMacrophages/immunology*MiceMice, Inbred BALB CNitric Oxide Synthase/geneticsNitric Oxide Synthase/metabolismNitric Oxide Synthase Type IINitrites/metabolismRNA, Messenger/geneticsRNA, Messenger/metabolismReverse Transcriptase Polymerase Chain ReactionSolubilityViral Envelope Proteins/antagonists & inhibitorsViral Envelope Proteins/chemistryViral Envelope Proteins/immunology*
PMID
11600202
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
강, 엽
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse