13 252

Cited 0 times in

Process extension and intracellular Ca2+ in cultured murine oligodendrocytes

Yoo, AS; Krieger, C; Kim, SU
Brain research, 827(1-2):19-27, 1999
Journal Title
Brain research
Previous investigations have shown that phorbol esters stimulate process extension in oligodendrocytes (OL), likely by the activation of protein kinase C (PKC). In this report, we demonstrate that treatment of OL with 4beta-phorbol-12, 13-dibutyrate (PDB; 0.1-1 microM) resulted in an increase in intracellular Ca2+ concentration ([Ca2+]i) from 94+/-2 nM (mean+/-S.E.M.) to 244+/-10 nM. This increase was produced by Ca2+ influx through a La3+-insensitive pathway. Changes in [Ca2+]i were also produced by modifying the extracellular Ca2+ concentration ([Ca2+]o) where [Ca2+]i was increased by elevations in [Ca2+]o. In parallel experiments we found that increased [Ca2+]o alone, without concurrent phorbol ester application, resulted in increased OL process extension as determined by the percent of OL with long processes (greater than 3 times the cell body diameter). These results demonstrate that increasing [Ca2+]o stimulates OL process outgrowth. Furthermore, both elevations in [Ca2+]o and PDB exposure increase [Ca2+]i, suggesting that some of the effects of phorbol esters on OL process extension are likely mediated by changes in [Ca2+]i.
MeSH terms
AnimalsBiological Transport/drug effectsBiological Transport/physiologyCalcium/analysisCalcium/metabolism*Calcium Channels/metabolismCarcinogens/pharmacologyCell Division/drug effectsCells, CulturedChelating Agents/pharmacologyEgtazic Acid/analogs & derivativesEgtazic Acid/pharmacologyEnzyme Inhibitors/pharmacologyFluorescent DyesFura-2Indoles/pharmacologyLanthanum/pharmacologyMiceMice, Inbred BALB COligodendroglia*/chemistryOligodendroglia*/cytologyOligodendroglia*/enzymologyPhorbol 12,13-Dibutyrate/pharmacologyProtein Kinase C/antagonists & inhibitorsProtein Kinase C/metabolism
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
Full Text Link
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.