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Process extension and intracellular Ca2+ in cultured murine oligodendrocytes

Authors
Yoo, AS; Krieger, C; Kim, SU
Citation
Brain research, 827(1-2):19-27, 1999
Journal Title
Brain research
ISSN
0006-89931872-6240
Abstract
Previous investigations have shown that phorbol esters stimulate process extension in oligodendrocytes (OL), likely by the activation of protein kinase C (PKC). In this report, we demonstrate that treatment of OL with 4beta-phorbol-12, 13-dibutyrate (PDB; 0.1-1 microM) resulted in an increase in intracellular Ca2+ concentration ([Ca2+]i) from 94+/-2 nM (mean+/-S.E.M.) to 244+/-10 nM. This increase was produced by Ca2+ influx through a La3+-insensitive pathway. Changes in [Ca2+]i were also produced by modifying the extracellular Ca2+ concentration ([Ca2+]o) where [Ca2+]i was increased by elevations in [Ca2+]o. In parallel experiments we found that increased [Ca2+]o alone, without concurrent phorbol ester application, resulted in increased OL process extension as determined by the percent of OL with long processes (greater than 3 times the cell body diameter). These results demonstrate that increasing [Ca2+]o stimulates OL process outgrowth. Furthermore, both elevations in [Ca2+]o and PDB exposure increase [Ca2+]i, suggesting that some of the effects of phorbol esters on OL process extension are likely mediated by changes in [Ca2+]i.
MeSH terms
AnimalsBiological Transport/drug effectsBiological Transport/physiologyCalcium/analysisCalcium/metabolism*Calcium Channels/metabolismCarcinogens/pharmacologyCell Division/drug effectsCells, CulturedChelating Agents/pharmacologyEgtazic Acid/analogs & derivativesEgtazic Acid/pharmacologyEnzyme Inhibitors/pharmacologyFluorescent DyesFura-2Indoles/pharmacologyLanthanum/pharmacologyMiceMice, Inbred BALB COligodendroglia*/chemistryOligodendroglia*/cytologyOligodendroglia*/enzymologyPhorbol 12,13-Dibutyrate/pharmacologyProtein Kinase C/antagonists & inhibitorsProtein Kinase C/metabolism
PMID
10320689
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
AJOU Authors
김, 승업
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