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Suicide Gene Therapy for Bladder Cancer Using a Recombinant Adenovirus Expressing Escherichia Coli Cytosine Deaminase

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dc.contributor.author안, 미원-
dc.contributor.author임, 호영-
dc.contributor.authorKao, Chinghai-
dc.contributor.authorGardner, Thomas A.-
dc.contributor.author고, 성주-
dc.contributor.author김, 세중-
dc.date.accessioned2011-11-23T04:52:46Z-
dc.date.available2011-11-23T04:52:46Z-
dc.date.issued2003-
dc.identifier.issn0494-4747-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/4517-
dc.description.abstractPurpose: The poor prognosis of advanced bladder cancer requires the investigation of novel treatment modalities. In this study, we investigated the suicide gene therapy for bladder cancer, using the adenovirus-mediated expression of Escherichia coli cytosine deaminase(CD) in conjunction with the prodrug 5-fluorocytosine(5-FC). Materials and Methods: A replication-deficient recombinant adenovirus, which contains the Rous sarcoma virus(RSV) promoter driving the expression of CD,(Ad-RSV-CD) was constructed. In vitro cell-killing assay, using Ad-RSV-CD(20 MOI) plus 5-FC(500mug), was performed in bladder cancer cell lines, HT-1376, UM-UC-3 and NBT-II. The CD enzymatic activity was measured in the Ad-RSV-CD(20 MOI) infected cells, and the concentrations of 5-fluorouracil(5-FU) yielding an IC50 were calculated for those cells. Results: 5-FU dose response curve showed that IC50 of NBT-II was 0.8mug, HT-1376 1.0mug and UM-UC-3 5.1mug at day 6. The CD enzymatic activities of the Ad-RSV-CD infected UM-UC-3, HT-1376 and NBT-II cells were 5696, 4655, 1766 pmole/1x106 cells, respectively. Whereas the administration of 5-FC(500mug) or Ad-RSV-CD(20 MOI) alone demonstrated no cytotoxicity to cells, Ad-RSV-CD/5-FC exhibited a significant cytotoxic effect in the cells, especially the UM-UC-3 and HT-1376. Conclusions: Ad-RSV-CD/5-FC suicide gene therapy is effective for bladder cancer cells in cell cultures, suggesting this approach may have potential as a strategy for the treatment of bladder cancer.en
dc.formattext/plain-
dc.language.isoko-
dc.titleSuicide Gene Therapy for Bladder Cancer Using a Recombinant Adenovirus Expressing Escherichia Coli Cytosine Deaminase-
dc.title.alternative대장균 사이토신탈아미노효소 발현 재조합 아데노바이러스를 이용한 방광암의 자살유전자치료-
dc.typeArticle-
dc.identifier.urlhttp://pdf.medrang.co.kr/Kju/044/Kju044-03-07.pdf-
dc.subject.keywordBladder cancer-
dc.subject.keywordSuicide gene therapy-
dc.subject.keywordCytosine deaminase-
dc.subject.keywordAdenovirus-
dc.contributor.affiliatedAuthor임, 호영-
dc.contributor.affiliatedAuthor김, 세중-
dc.type.localJournal Papers-
dc.citation.titleKorean journal of urology-
dc.citation.volume44-
dc.citation.number3-
dc.citation.date2003-
dc.citation.startPage244-
dc.citation.endPage249-
dc.identifier.bibliographicCitationKorean journal of urology, 44(3). : 244-249, 2003-
dc.relation.journalidJ004944747-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
Journal Papers > School of Medicine / Graduate School of Medicine > Urology
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