Increased Expression of Phospholipase C-γ1 in Human Gastric Cancer Tissues
Hwang, SC; Ha, MJ; Park, KH; Joo, HJ; Cho, YK
Ajou medical journal, 1(1):68-75, 1996
Ajou medical journal; 아주의학
Phospholipase C (PLC) plays a central role in signal transduction and is important in cellular growth, differentiation and transformation. There are currently ten known mammalian isozymes of PLC reported to date. However, their biological implication in carcinogenesis and in disease is not fully understood.
Here we examined the relative content of various PLC isozymes present in 50 paired surgical specimens from human gastric cancer patients. Western blot analysis demonstrated considerablely higher levels of PLC-gl protein in gastric cancer tissues from 41 of 50 specimens as compared to that of normal mucosa. On immunohistochemistry, distinct speckles or clumps of PLC-gl staining were observed in the cytoplasms of primary malignant lesions as well as the invading cancer cell nests, in contrast to the normal tissues which were negative. Little differences in other isozymes were found. Only PLC-d1 was slightly increased in about two thirds of the patients, however, to a much lesser extent.
In conclusion, although the causes of increased PLC-gl in gastric carcinomas remain to be explored further, the elevated level of PLC-gl suggests an altered signal transduction pathways in gastric carcinomas and may indicate an important role played by PLC-g1 in pathogenesis and proliferation of cancer cells.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :2,787,207
Total Download :1,136,409
Today View :890
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.