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E1B-19k does not Localize in Mitochondria nor Dimerize Bax even with the Staurosporine

Other Title
E1B-19k의 세포내 위치와 Bax와의 Dimerization에 관한 연구
Authors
윤, 수한; 김, 진영; 박, 승우; 안, 영환; 안, 영민; 조, 기홍; 조, 경기
Citation
Taehan Sinkyŏng Oekwa Hakhoe chi, 29(6):725-730, 2000
Journal Title
Taehan Sinkyŏng Oekwa Hakhoe chi; Journal of Korean Neurosurgical Society; 대한신경외과학회지
ISSN
1225-8245
Abstract
Purpose:The subcellular localization of E1B-19k has been known cytosol or nuclear membrane by immuno-histochemical staining and could dimerize with Bax to regulate cell death also known by the in-vitro immunoprecipitation. We planed to confirm this dimerization of E1B-19k with Bax in vivo in Cos-7 cells by using green fluorescent protein.



Material and Method:We cloned E1B-19k and Bax into C3-EGFP. C3-EGFP-E1B-19k, C3-EGFP-Bax, and C3-EGFP-E1B-19k and pcDNA3-Bax were transfected into Cos-7 cells. We explored location of E1B-19k and Bax, and confirmed its dimerization with Bax in transfected living healthy Cos-7 cells by following green fluorescent protein of E1B-19k on the confocal microscope.



Results:E1B-19k was located diffusely in cytoplasm and in nucleus but not in mitochondria. It prevented cell death from the apoptosis by staurosporine but its location was not changed. GFP-E1B-19k is not changed its intracellular location with Bax even with staurosporine.



Conclusion:These results support that E1B-19k does not localize in mitochondria nor dimerize with Bax even with staurosporine. We could anticipate E1B-19k prevent cell death via the other dimerizing partner or pathways.
Keywords
E1B-19kBaxDimerizationApoptosisStaurosporineGreen fluorescent protein
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Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
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