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Effect of Transforming Growth Factor (TGF)-β and Peroxisome Proliferator-Activated Receptor (PPAR)-gamma in Endometrial Decidualization

Other Title
자궁내막 탈락막화에서 Transforming growth factor (TGF)-β와 Peroxisome proliferator-activated receptor (PPAR)-gamma가 미치는 영향
Authors
권, 종희; 이, 재훈; 장, 혜진; 박, 동욱; 김, 영아; 김, 미란; 황, 경주
Citation
Taehan Sanbuinkwa Hakhoe chi, 49(6):1294-1303, 2006
Journal Title
Taehan Sanbuinkwa Hakhoe chi; Korean journal of obstetrics and gynecology; 대한산부인과학회지
ISSN
1738-5628
Abstract
Objective: To ascertain the expression of transforming growth factor (TGF)-β receptors in normal human endometrium during the menstrual cycle, and the action of TGF-β and peroxisome proliferator-activated receptor (PPAR)-gamma during endometrial decidualization using cultured human endometrial stromal cells. Methods: Human endometrial tissues were examined immunohistochemically for the expression of TGF-β receptors and Smad. Western blotting, confocal microscopy and viable cell counting were performed on cultured human endometrial stromal cells which were treated with TGF-β(10ng/mL) and PPAR-gamma agonists (Rosiglitazone(R) 50 nM). Thereafter we compared the effect of TGF-β and PPAR-gamma on the Smad phosphorylation, prolactin expression, and cellular proliferation in vitro human endometrial decidualization. Results: The results revealed significantly increased expression of both TGF-β receptor-I and -II proteins in the secretory stromal cells compared to the epithelial cells of human endometrium. The degree of expression and translocation into the nucleus of the phosphorylated Smad2/3 was also increased in the secretory endometrium compared to the proliferative endometrium. In the stromal cell culture, the decidualization process associated with TGF-β and pSmad is inhibited by the PPAR-gamma agonist. In contrast to the PPAR-gamma agonist, TGF-β inhibits cellular proliferation. Conclusion: TGF-β /Smad signaling pathway is essential for endometrial decidualization and closely related to cellular differentiation. PPAR-gamma plays a conflicting role by directly acting on the Smad protein and blocking the TGF-β /Smad signaling pathway.
Keywords
Endometrial stromal cellDecidualizationTGF-βSmadPPAR-gamma
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Obstetrics & Gynecology
AJOU Authors
장, 혜진김, 미란황, 경주
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