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Establishment of Three Dimensional in-vitro Culture System with Human Endometrial Cells : Induction of Differentiation by Sex Steroid Hormone & Characterization

Other Title
3 차원적 자궁내막 세포배양 체계의 확립 : 성선 호르몬에 의한 분화유도 및 특성
Authors
권, 혁찬; 박, 원일; 김, 선영; 박, 동욱; 김, 미란; 한, 성원; 김, 대운; 이, 호준; 홍, 서유; 박, 준영; 김, 세광; 조, 동제
Citation
Taehan Sanbuinkwa Hakhoe chapchi, 44(1):65-73, 2001
Journal Title
Taehan Sanbuinkwa Hakhoe chapchi; Korean journal of obstetrics and gynecology; 대한산부인과학회잡지
ISSN
0494-4755
Abstract
Objective : The aim of this study was to establish three-dimensionally cultured endometrial cell model containing endometrial stromal cell (ESC), endometrial epithelial cell (EEC) and extracellular matrix (ECM) and to compare the morphological and biomolecular expression patterns of this model with mid-luteal endometrium in vivo. Materials and Methods : The EEC and ESC was obtained from hysterectomy specimen and cultured separately. The EEC was overlayered in Matrigel layer on ESC embedded in collagen. The model had been cultured for 48 h in DMEM medium containing estrogen and progesterone. The ultrastructure was evaluated by electron microscopy. The expression of integrins, cyclooxygenases and matrix metalloproteinases were examined by immunohistochemistry and zymography. Results : EEC in three-dimensional culture model grew with polarity and tight junction and desmosome between cells were found. The formation of pinopodes was also detected. In three-dimensionally cultured endometrial cell model, the expression of integrin α1, α4, β3, MMP-1, -2, -3 and 9 was detected which was not expressed in monolayer culture of EEC, ESC or ESC embedded in collagen. Conclusion : The three-dimensionally cultured endometrial cell model possessed the morphological and biomolecular characteristics of in vivo endometrium of implantation period. These characteristics could be achieved by paracrine interactions between ESC and EEC. This model may contribute to the studies of differentiation of endometrium, process of implantation and pathophysiology of implantation-related diseases.
Keywords
Cervix cancerLymphoepitheliomalike carcinomaLymphocyte
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Obstetrics & Gynecology
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