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Comparative Study of ImmunoCAP and ELISA for Detecting Allergen Specific IgE to Japanese Hop Pollen and Two Spotted Spider Mite

Other Title
알레르기 환자에서 ImmunoCAP 시스템을 이용한 환삼덩굴 및 점박이응애황원에 대한 특이 IgE치 측정: 피부반응도 및 ELISA 결과와의 비교
Authors
김, 현아; 예, 영민; 허, 규영; 김, 승현; 서, 창희; 남, 동호; 박, 해심
Citation
Chʿŏnsik mit alrerugi, 26(2):129-135, 2006
Journal Title
Chʿŏnsik mit alrerugi; Journal of asthma, allergy and clinical immunology; Korean journal of asthma, allergy and clinical immunology; 천식 및 알레르기
ISSN
1226-8739
Abstract
Background & Objectives: Japanese hop pollen (Hop J) and two spotted spider mite (TSM) are common allergens in this country. The aim of this study was to validate a new immunoCAP test for detecting allergen-specific IgE to Hop J and TSM compared to skin reactivity and ELISA results.



Methods: Ninty-five allergy patients showing more than 2+ response on skin prick test to Hop J, 131 allergy patients showing more than 2+ response on skin prick test to TSM and 60 non-atopic healthy controls were enrolled. Skin prick testing was performed using homemade Hop J pollen and TSM extracts. The specific IgE levels to both homemade allergens were measured by ELISA, which were compared to those of the immunoCAP system.



Results: The positive rates of serum specific IgE to Hop J were 57% by ELISA and 51% by Pharmacia immunoCAP system among 95 patients tested. The positive rates of serum specific IgE to TSM were 28% by ELISA and 50% by immunoCAP system among 131 patients tested. A significant correlation was found in specific IgE level between ELISA and the immunoCAP system (r=0.63 for Hop J and r=0.61 for TSM, P<0.05).



Conclusion: The new immunoCAP system for detecting serum specific IgE antibodies to Hop J pollen was comparable to the results of other in vivo and in vitro test and can be applied for the diagnosis of Hop J-allergic patients. New immunoCAP system to TSM was superior to the ELISA test, and can be used for screening TSM-sensitive patients.
Keywords
Japanese Hop PollenTwo Spotted Spider MiteImmunoCAP system
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > Research Organization > Regional Clinical Trial Center
Journal Papers > School of Medicine / Graduate School of Medicine > Rheumatology
AJOU Authors
예, 영민허, 규영김, 승현서, 창희남, 동호박, 해심
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