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Prevalence of Ambler Class A Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea

DC Field Value Language
dc.contributor.author강, 지혜-
dc.contributor.author배, 일권-
dc.contributor.author권, 수봉-
dc.contributor.author정, 석훈-
dc.contributor.author이, 종욱-
dc.contributor.author이, 위교-
dc.contributor.author강, 정옥-
dc.contributor.author안, 지영-
dc.contributor.author홍, 성근-
dc.contributor.author신, 종희-
dc.contributor.author어, 영-
dc.contributor.author박, 연준-
dc.contributor.author김, 의종-
dc.contributor.author이, 경원-
dc.contributor.author용, 동은-
dc.contributor.author우, 건조-
dc.description.abstract배경:본 연구에서는 국내에서 분리되는 Escherichia coli와 Klebsiella pneumoniae의 Ambler class A extended-spectrum β-lactamase (ESBL) 생성 현황을 알아보고자 하였다.

방법:2003년 2월부터 7월까지 전국 12개 병원에서 분리된 E. coli와 K. pneumoniae를 수집하였다. 항균제 감수성은 디스크 확산법으로 시험하였고, ESBL 생성은 double-disk synergy 시험으로 확인하였다. 한천희석법으로 β-lactam 항균제의 최소억제 농도를 측정하였다. 중합연쇄반응으로 TEM형, SHV형, CTX-M형, PER-1형, VEB형, IBC형, GES형 및 TLA형 유전자를 검출하였고, 중합연쇄반응 산물의 염기서열을 양방향으로 분석하였다.

결과:국내 12개 병원에서 수집한 E. coli와 K. pneumoniae의 ceftazidime에 대한 내성율은 각각 8.5%와 20.1%이었다. E. coli에서 가장 흔한 Ambler class A ESBL은 CTX-M-15 (4주)와 CTX-M-3 (3주)였으며, CTXM-14, SHV-12 및 TEM-52 유전자를 지닌 균주 각 1주씩 검출되었다. K. pneumoniae에서 가장 흔한 ESBL은 SHV-12 (30주)와 CTX-M-3 (13주)였으며, CTX-M-14 (5주), SHV-2a (3주), SHV-5 (2주), TEM-52 (1주) 및 GES-3 (2주) 유전자를 지닌 균주도 검출되었다.

결론:본 연구를 통하여 CTX-M형 ESBL을 생성하는 E. coli와 K. pneumoniae가 국내에서 확산중이며 GES-3형 ESBL의 출현을 확인할 수 있었다.

Background:The aim of this study is to determine the nationwide prevalence of Ambler class A extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and to characterize genotypes of ESBLs.

Methods:During the period of February through July, 2003, E. coli and K. pneumoniae isolates were collected from 12 hospitals in Korea. Antimicrobial susceptibilities were tested by disk diffusion method, and ESBL-production was determined by the double-disk synergy test. MICs of β-lactam antibiotics were tested by agar dilution method. Searches for blaTEM, blaSHV, blaCTX-M, blaPER-1, bla-VEB, blaIBC, blaGES and blaTLA genes were performed by PCR amplification, and the genotypes of ESBLs were determined by direct nucleotide sequence analysis of amplified products.

Results:Resistance rates of E. coli (n=246) and K. pneumoniae (n=239) isolates to ceftazidime were 8.5% and 20.1%, respectively. Most prevalent Ambler class A ESBL genotypes in E. coli isolates were blaCTX-M-15 (n=4) and blaCTX-M-3 (n=3), and each of blaCTX-M-14, blaSHV-12, and blaTEM-52 gene was also found in one isolate. Most prevalent ESBL genotypes in K. pneumoniae were blaSHV-12 (n=30) and blaCTX-M-3 (n=13), and blaCTX-M-14 (n=5). blaSHV-2a (n=3), blaSHV-5 (n=2), blaTEM-52 (n=1), blaGES-3 (n=2) genes were also found.

Conclusion:CTX-M-type ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.
dc.titlePrevalence of Ambler Class A Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea-
dc.title.alternativeAmbler Class A Extended-Spectrum β-Lactamase 생성Escherichia coli와 Klebsiella pneumoniae의 국내 분리 현황-
dc.subject.keywordExtended-spectrum β-lactamase-
dc.subject.keywordE. coli-
dc.subject.keywordK. pneumoniae-
dc.type.localJournal Papers-
dc.citation.titleKorean journal of clinical microbiology-
dc.identifier.bibliographicCitationTaehan Imsang Misaengmul Hakhoe chi, 8(1):17-25, 2005-
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Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
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