The Oxidative Stress and the Antioxidant System in Type 2 Diabetics with Complications
만성합병증을 동반한 제2형 당뇨병 환자에서 산화 스트레스와 항산화 방어체계
하, 애화; 노, 혜림; 정, 윤석; 이, 관우; 김, 현만; 조, 정순
The journal of Korean diabetes association, 22(3):253-261, 1998
The journal of Korean diabetes association; 당뇨병
Background: Diabetes mellitus represents a state of increased oxidative stress which is
based on the evidence of increased peroxidation and glycosylation, and reduced
antioxidant system. It has been suggested that increased oxidative stress may play an
important role on the pathogenesis of diabetic complication in type 2 diabetes. However, limited informations regarding the oxidative stress and antioxidant system in diabetic complications are available. Therefore the purpose of this study is to determine the oxidative stress and antioxidant system in type 2 diabetes with diabetic complications.
Methods: The study population consisted of 94 type 2 diabetic patients and 44 normal
subjects. The concentration of thiobarbituric acid reactive substance(TBA-RS) and the activities of antioxidants enzymes, catalase, superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) in erythrocyte were determined by using spectrophotometer. The plasma concentrations of β-carotene, lycopene, lutein, α-tocopherol and retinol were determined by using HPLC.
Results: The TBA-RS concentrations in type 2 diabetes(1.33土0.30nmo1/mL) were
significantly higher than those in normal subjects(1.10±0.17nmo1/mL). Also the
TBA-RS concentrations between subjects with complications(1.37±0.27nmo1/mL) and
without complications(1.28±0.17umo1/mL) differed (p<0.05). The activities of SOD and
GSH-Px in type 2 diabetes(2.99±0.80U/ingHb, 2.88±0.39u/mgHb) were significantly
lower than those in normal subjects(3.54±0.44U/mgHb, 3.14±0.39U/mgHb). GSH-Px
between diabetics with(2.81±0.6U/mgHb) and without complications(3.17±0.4U/mgHb)
differed significantly. The plasma concentrations of lycopene and β-carotene were significantly lower in type 2 diabetes(0.07±0.05umo1/L, 0.54±0.27umo1/L) than in control subjects(0.14±0.06umo1/L, 0.67±0.32umol/L). Also, lycopene and β-carotene in subjects with complications(0.05±0.04umo1/L, 0.45±0.23umol/L) were lower than in subjects without complications(0.08±0.05umo1/L, 0.62±0.300umo1/L). No significant differences in plasma α-tocopherol concentrations between subjects with and without complications(19.42±0.93umo1/L vs 18.66±0.79umo1/L).
Conclusion: This study showed that in diabetes with diabetic complications, the lipid peroxidation of erythrocytes are highly increased and the antioxidant reserves are significantly depleted, compared with diabetes without diabetic complications, which suggests that diabetes with complications are under high oxidative stress and the supplementations of carotenoids could decrease the oxidative stress in diabetes with diabetic complications.
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