22 277

Cited 6 times in

Effects of immunization with the rNfa1 protein on experimental Naegleria fowleri-PAM mice

Authors
Lee, YJ; Kim, JH; Sohn, HJ; Lee, J; Jung, SY; Chwae, YJ; Kim, K; Park, S; Shin, HJ
Citation
Parasite immunology, 33(7):382-389, 2011
Journal Title
Parasite immunology
ISSN
0141-98381365-3024
Abstract
Free-living Naegleria fowleri causes primary amoebic meningoencephalitis (PAM) in humans and animals. To examine the effect of immunization with Nfa1 protein on experimental murine PAM because of N. fowleri, BALB/c mice were intra-peritoneally or intra-nasally immunized with a recombinant Nfa1 protein. We analysed Nfa1-specific antibody and cytokine induction, and the mean survival time of infected mice. Mice immunized intra-peritoneally or intra-nasally with rNfa1 protein developed specific IgG, IgA and IgE antibodies; the IgG response was dominated by IgG1, followed by IgG2b, IgG2a and IgG3. High levels of the Th1 cytokine, IFN-γ, and the regulatory cytokine, IL-10, were also induced. The mean survival time of mice immunized intra-peritoneally with rNfa1 protein was prolonged compared with controls, (25.0 and 15.5 days, respectively). Similarly, the mean survival time of mice immunized intra-nasally with rNfa1 protein was 24.7 days, compared with 15.0 days for controls.
MeSH terms
Administration, IntranasalAnimalsAntibodies, Protozoan/bloodAntigens, Protozoan/genetics/*immunologyCentral Nervous System Protozoal Infections/immunology/*prevention & controlCytokines/secretionDisease Models, AnimalFemaleImmunoglobulin A/bloodImmunoglobulin E/bloodImmunoglobulin G/bloodInjections, IntraperitonealLeukocytes, Mononuclear/immunologyMiceMice, Inbred BALB CNaegleria fowleri/*immunology/pathogenicityProtozoan Proteins/genetics/*immunologyProtozoan Vaccines/administration & dosage/genetics/*immunologyRodent Diseases/prevention & controlSpleen/immunologySurvival AnalysisVaccines, Synthetic/administration & dosage/genetics/immunology
DOI
10.1111/j.1365-3024.2011.01296.x
PMID
21535019
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
김, 종현최, 용준김, 경민박, 선신, 호준
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse