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Effects of immunization with the rNfa1 protein on experimental Naegleria fowleri-PAM mice

Lee, YJ; Kim, JH; Sohn, HJ; Lee, J; Jung, SY; Chwae, YJ; Kim, K; Park, S; Shin, HJ
Parasite immunology, 33(7):382-389, 2011
Journal Title
Parasite immunology
Free-living Naegleria fowleri causes primary amoebic meningoencephalitis (PAM) in humans and animals. To examine the effect of immunization with Nfa1 protein on experimental murine PAM because of N. fowleri, BALB/c mice were intra-peritoneally or intra-nasally immunized with a recombinant Nfa1 protein. We analysed Nfa1-specific antibody and cytokine induction, and the mean survival time of infected mice. Mice immunized intra-peritoneally or intra-nasally with rNfa1 protein developed specific IgG, IgA and IgE antibodies; the IgG response was dominated by IgG1, followed by IgG2b, IgG2a and IgG3. High levels of the Th1 cytokine, IFN-γ, and the regulatory cytokine, IL-10, were also induced. The mean survival time of mice immunized intra-peritoneally with rNfa1 protein was prolonged compared with controls, (25.0 and 15.5 days, respectively). Similarly, the mean survival time of mice immunized intra-nasally with rNfa1 protein was 24.7 days, compared with 15.0 days for controls.
MeSH terms
Administration, IntranasalAnimalsAntibodies, Protozoan/bloodAntigens, Protozoan/genetics/*immunologyCentral Nervous System Protozoal Infections/immunology/*prevention & controlCytokines/secretionDisease Models, AnimalFemaleImmunoglobulin A/bloodImmunoglobulin E/bloodImmunoglobulin G/bloodInjections, IntraperitonealLeukocytes, Mononuclear/immunologyMiceMice, Inbred BALB CNaegleria fowleri/*immunology/pathogenicityProtozoan Proteins/genetics/*immunologyProtozoan Vaccines/administration & dosage/genetics/*immunologyRodent Diseases/prevention & controlSpleen/immunologySurvival AnalysisVaccines, Synthetic/administration & dosage/genetics/immunology
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
김, 종현최, 용준김, 경민박, 선신, 호준
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