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Selective 3,4-Dihydroxyphenylalanine Analysis in Human Urine as Ethoxycarbonyl-tert-butyldimethylsilyl Derivatives by Gas Chromatography-Mass Spectrometry.

Authors
Paik, MJ; Nguyen, DT; Yoon, J; Cho, IS; Shim, W; Kim, KR; Cho, KH; Choi, S; Lee, G
Citation
Bulletin of the Korean Chemical Society, 32(3):977-980, 2011
Journal Title
Bulletin of the Korean Chemical Society
ISSN
0253-29641229-5949
Abstract
A new analytical method for measurement of 3,4-dihydroxyphenylalanine (DOPA) in human urine was developed. DOPA from an aqueous solution was converted into an ethoxycarbonyl (EOC) derivative. A tert-butyldimethylsilyl (TBDMS) reaction under anhydrous conditions was then attempted for analysis by gas chromatography-mass spectrometry in selected ion monitoring mode. A new mass spectral data on DOPA as a tri-EOC/mono-TBDMS derivative was built. This method showed good linearity (r ≥ 0.999), precision (% relative standard deviation = 3.1-9.2), and accuracy (% relative error = −7.2-8.8), with a detection limit of 0.05ng/mL. This selective and accurate method of DOPA analysis will be useful for biochemical monitoring of various neurological disorders including Parkinson’s disease in biological fluids.
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Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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