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Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity.

Authors
Song, KJ; Song, KH; Kim, JH; Sohn, HJ; Lee, YJ; Park, CE; Shin, HJ
Citation
Parasitology research, 103(2):313-317, 2008
Journal Title
Parasitology research
ISSN
0932-01131432-1955
Abstract
To evaluate the role of heat shock 70 protein (HSP70) in free-living amoeba, a constitutive and inducible heat shock 70 gene of pathogenic Naegleria fowleri has previously been cloned, characterized, and named as Nf-cHSP70. The Nf-cHSP70 is localized in the cytoplasm, pseudopodia, and phagocytic food-cups. To investigate the role of Nf-cHSP70 in the pathogenicity of N. fowleri, the synthesis of N. fowleri HSP70 was first inhibited with benzylidene lactam compound (KNK437), and Nf-cHSP70 gene was knock-downed with antisense oligomers, which were designed with a start region-specific antisense oligonucleotides (24 oligomers) and modified with phosphorothioate. KNK437 inhibited the induction of N. fowleri HSP70 in a dose-dependent manner. In addition, 300 muM KNK437 reduced the proliferation of N. fowleri to 79.4% of untreated control (100%). Nf-cHSP70 knock-downed N. fowleri with antisense oligomers showed 68.5% reduction of proliferation in comparison with untreated control (100%). The cytotoxicity of N. fowleri against CHO target cells was reduced to 42.1% by KNK437 and 68.6% by antisense oligomers. These results suggest that the cloned Nf-cHSP70 plays an important role in the proliferation and cytotoxicity of pathogenic N. fowleri.
MeSH terms
AnimalsBenzhydryl Compounds/pharmacologyCHO Cells/parasitologyCricetinaeCricetulusGene DeletionHSP70 Heat-Shock Proteins/antagonists & inhibitorsHSP70 Heat-Shock Proteins/geneticsHSP70 Heat-Shock Proteins/metabolism*Naegleria fowleri/growth & development*Naegleria fowleri/pathogenicity*Naegleria fowleri/physiologyProtozoan Proteins/geneticsProtozoan Proteins/metabolismPyrrolidinones/pharmacologyTrophozoites/growth & development
DOI
10.1007/s00436-008-0972-x
PMID
18421477
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Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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