41 297

Cited 13 times in

Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity.

Song, KJ; Song, KH; Kim, JH; Sohn, HJ; Lee, YJ; Park, CE; Shin, HJ
Parasitology research, 103(2):313-317, 2008
Journal Title
Parasitology research
To evaluate the role of heat shock 70 protein (HSP70) in free-living amoeba, a constitutive and inducible heat shock 70 gene of pathogenic Naegleria fowleri has previously been cloned, characterized, and named as Nf-cHSP70. The Nf-cHSP70 is localized in the cytoplasm, pseudopodia, and phagocytic food-cups. To investigate the role of Nf-cHSP70 in the pathogenicity of N. fowleri, the synthesis of N. fowleri HSP70 was first inhibited with benzylidene lactam compound (KNK437), and Nf-cHSP70 gene was knock-downed with antisense oligomers, which were designed with a start region-specific antisense oligonucleotides (24 oligomers) and modified with phosphorothioate. KNK437 inhibited the induction of N. fowleri HSP70 in a dose-dependent manner. In addition, 300 muM KNK437 reduced the proliferation of N. fowleri to 79.4% of untreated control (100%). Nf-cHSP70 knock-downed N. fowleri with antisense oligomers showed 68.5% reduction of proliferation in comparison with untreated control (100%). The cytotoxicity of N. fowleri against CHO target cells was reduced to 42.1% by KNK437 and 68.6% by antisense oligomers. These results suggest that the cloned Nf-cHSP70 plays an important role in the proliferation and cytotoxicity of pathogenic N. fowleri.
MeSH terms
AnimalsBenzhydryl Compounds/pharmacologyCHO Cells/parasitologyCricetinaeCricetulusGene DeletionHSP70 Heat-Shock Proteins/antagonists & inhibitorsHSP70 Heat-Shock Proteins/geneticsHSP70 Heat-Shock Proteins/metabolism*Naegleria fowleri/growth & development*Naegleria fowleri/pathogenicity*Naegleria fowleri/physiologyProtozoan Proteins/geneticsProtozoan Proteins/metabolismPyrrolidinones/pharmacologyTrophozoites/growth & development
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
신, 호준
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.