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A MAP kinase pathway is implicated in the pseudohyphal induction by hydrogen peroxide in Candica albicans.

Srinivasa, K; Kim, J; Yee, S; Kim, W; Choi, W
Molecules and cells, 33(2):183-193, 2012
Journal Title
Molecules and cells
Hydrogen peroxide (H(2)O(2)) functions as a ubiquitous intracellular messenger besides as an oxidative stress molecule. This dual role is based on the distinct cellular responses against different concentrations of H(2)O(2). Previously, we demonstrated that both low (> 1 mM) and high (4-10 mM) doses of exogenous H(2)O(2) induce filamentous growth with distinct cell morphology and growth rate in Candida albicans, suggesting the different transcription response. In this study, we revealed that the sub-toxic and toxic levels of H(2)O(2) indeed induced pseudohyphae, but not true hyphae. Supporting this, several hyphae-specific genes that are expressed in true hyphae induced by serum were not detected in either sub-toxic or toxic H(2)O(2) condition. A DNA microarray analysis was conducted to reveal the transcription profiles in cells treated with sub-toxic and toxic conditions of H(2)O(2). Under the sub-toxic condition, a small number of genes involved in cell proliferation and metabolism were up-regulated, whereas a large number of genes were up-regulated in the toxic condition where the genes required for growth and proliferation were selectively restricted. For pseudohyphal induction by sub-toxic H(2)O(2), Cek1 MAPK activating the transcription factor Cph1 was shown to be important. The absence of expression of several hyphae-specific genes known to be downstream targets of Cph1-signaling pathway for true hyphae formation suggests that the Cek1-mediated signaling pathway is not solely responsible for pseudohyphal formation by subtoxic H(2)O(2) and, but instead, complex networking pathway may exists by the activation of different regulators.
MeSH terms
Candida albicans/enzymology/genetics/*growth & developmentCell ProliferationFungal Proteins/genetics/*metabolismGene Expression ProfilingHydrogen Peroxide/chemistry/pharmacologyHyphae/enzymology/genetics/*growth & development*MAP Kinase Signaling SystemMitogen-Activated Protein Kinase 3/genetics/*metabolismOligonucleotide Array Sequence AnalysisOxidative Stress/geneticsTranscription Factors/genetics/*metabolism
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Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
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김, 완기
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