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Chiral separation of N-methyl-DL-aspartic acid in rat brain tissue as N-ethoxycarbonylated (S)-(+)-2-octyl ester derivatives by GC-MS

Authors
Nguyen, DT; Kim, KR; Lee, G; Paik, MJ
Citation
Biomedical chromatography : BMC, 26(11):1353-1356, 2012
Journal Title
Biomedical chromatography : BMC
ISSN
0269-38791099-0801
Abstract
A selective and sensitive analytical method was developed for enantiomeric separation and determination of N-methyl-DL-aspartic acid (NMA). The method involved the conversion of each enantiomer into N-ethoxycarbonylated (S)-(+)-2-octyl ester derivative for the direct separation by gas chromatography-mass spectrometry (GC-MS). The diastereomeric derivatives showed characteristic mass spectral properties for analysis by selected ion monitoring mode (SIM) and enabling enantioseparation on an achiral capillary column. Two enantiomers were baseline separated, and the detection limits for N-methyl-L-aspartic acid (NMLA) and N-methyl-D-aspartic acid (NMDA) were 0.07 and 0.03 ng/g, respectively. When applied to rat brain tissues for absolute configuration of NMA, only NMDA was determined, while NMLA was monitored as lower than the limit of detection.
MeSH terms
Animals*Brain ChemistryEsters/*chemistry/*isolation & purificationGas Chromatography-Mass Spectrometry/*methodsLimit of DetectionN-Methylaspartate/*analogs & derivatives/chemistry/isolation & purificationRatsReproducibility of ResultsStereoisomerism
DOI
10.1002/bmc.2703
PMID
22290726
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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