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Protective effect of grape seed extract against oxidative stress-induced cell death in a staurosporine-differentiated retinal ganglion cell line

Authors
Yang, H; Lee, BK; Kook, KH; Jung, YS; Ahn, J
Citation
Current eye research, 37(4):339-344, 2012
Journal Title
Current eye research
ISSN
0271-36831460-2202
Abstract
OBJECTIVE: Grape seed extract (GSE) is a potent antioxidant. We examined the effect of GSE on oxidative stress-induced cell death in a transformed retinal ganglion cell line, RGC-5.



METHODS: Staurosporine-differentiated RGC-5 (ssdRGC-5) cells obtained by treating RGC-5 cells with 1 µM staurosporine were incubated with GSE for 2 h and then exposed to buthionine sulfoximine plus glutamate (B/G) for 24 h. Cell death was detected using the LIVE/DEAD viability assay and the type of cell death was evaluated using fluorescein isothiocyanate-conjugated Annexin-V/propidium iodide staining. To investigate the mechanism underlying cell death, we determined the caspase-3 activity and level of reactive oxygen species (ROS) formation.



RESULTS: Treatment of ssdRGC-5 cells with B/G increased intracellular ROS and induced apoptosis (not necrosis) with increasing caspase-3 activity. GSE rescued the ssdRGC-5 cells from oxidative stress-induced cell death by inhibiting both intracellular ROS production and caspase-3 activation.



CONCLUSION: GSE had a neuroprotective effect against oxidative stress-induced apoptotic death in ssdRGC-5 cells.
MeSH terms
Antioxidants/*pharmacologyCaspase 3/drug effects/metabolismCell Death/*drug effectsCell LineCulture MediaEnzyme Inhibitors/pharmacologyFluorometryGrape Seed Extract/*pharmacologyHumansIntracellular Fluid/metabolismOxidative Stress/*drug effectsReactive Oxygen Species/metabolismRetinal Ganglion Cells/drug effects/*pathologyStaurosporine/pharmacology
DOI
10.3109/02713683.2011.645106
PMID
22440165
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Ophthalmology
AJOU Authors
양, 홍석국, 경훈안, 재홍
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