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Pirfenidone inhibits transforming growth factor-β1-induced fibrogenesis by blocking nuclear translocation of Smads in human retinal pigment epithelial cell line ARPE-19

Authors
Choi, K; Lee, K; Ryu, SW; Im, M; Kook, KH; Choi, C
Citation
Molecular vision, 18:1010-1020, 2012
Journal Title
Molecular vision
ISSN
1090-0535
Abstract
PURPOSE: Transforming growth factor-β (TGF-β) plays a key role in transforming retinal pigment epithelial (RPE) cells into mesenchymal fibroblastic cells, which are implicated in proliferative vitreoretinopathy. Herein, we tested the effect of pirfenidone, a novel antifibrotic agent, on TGF-β1-mediated fibrogenesis in the human RPE cell line ARPE-19.



METHODS: The effect of pirfenidone on the TGF-β1-induced phenotype in ARPE-19 cells was measured with immunocytochemistry as the change in F-actin. Fibronectin and collagen production was measured with enzyme-linked immunosorbent assay, and cell migration activity was investigated using a scratch assay. Immunoblot analyses of cofilin, sma and mad protein (smad) 2/3, p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and extracellular signal-related kinase expression were conducted to elucidate the cell signaling networks that contribute to the antifibrotic effect of pirfenidone.



RESULTS: Treatment with TGF-β1 induced typical phenotypic changes such as formation of stress fiber running parallel to the long axis of cells and enhanced migration and production of extracellular matrix components such as collagen type I and fibronectin. This fibroblast-like phenotype induced by TGF-β1 was significantly inhibited by pretreatment with pirfenidone in a dose-dependent manner. We also elucidated the TGF-β signaling pathways as the target of the inhibitory effect of pirfenidone. Pirfenidone inhibited TGF-β signaling by preventing nuclear accumulation of active Smad2/3 complexes rather than phosphorylation of Smad2/3.



CONCLUSIONS: These results collectively provide a rational background for future evaluation of pirfenidone as a potential antifibrotic agent for treating proliferative vitreoretinopathy and other fibrotic retinal disorders.
MeSH terms
Actins/genetics/metabolismAnti-Inflammatory Agents, Non-Steroidal/*pharmacologyCell DifferentiationCell LineCell MovementCollagen/genetics/metabolismEpithelial Cells/cytology/*drug effects/metabolismExtracellular Signal-Regulated MAP Kinases/genetics/metabolismFibroblasts/cytology/metabolismFibronectins/genetics/metabolismFibrosis/prevention & controlGene Expression/drug effectsHumansJNK Mitogen-Activated Protein Kinases/genetics/metabolismProtein Transport/drug effectsPyridones/*pharmacologyRetinal Pigment Epithelium/cytology/*drug effects/metabolismSignal Transduction/drug effectsSmad Proteins/genetics/*metabolismTransforming Growth Factor beta1/*antagonists & inhibitors/pharmacologyVitreoretinopathy, Proliferative/drug therapy
PMID
22550395
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Ophthalmology
AJOU Authors
이, 기황국, 경훈
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