The purpose of this study is to present the serially rotated images (SRIs) of the dissected knee for the production of educational software of knee anatomy. In order to achieve the purpose, the SRIs of dissected knee were generated as follows. An embalmed knee was serially dissected to make eight layers of the dissected knee. The knee in each layer was serially rotated at every five degree angle and photographed to obtain 72 SRIs. In every SRI, all anatomical structures were annotated. The knee was serially flexed to make additional SRIs. Similar procedures were performed with a fresh knee. The SRIs of knee, prepared in this study, will be used as sources of the software, which will be helpful in educating knee anatomy.