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IL-6 induction of TLR-4 gene expression via STAT3 has an effect on insulin resistance in human skeletal muscle.

Authors
Kim, TH; Choi, SE; Ha, ES; Jung, JG; Han, SJ; Kim, HJ; Kim, DJ; Kang, Y; Lee, KW
Citation
Acta diabetologica, 50(2):189-200, 2013
Journal Title
Acta diabetologica
ISSN
0940-54291432-5233
Abstract
We investigated the cytokines and mechanisms involved in the induction of insulin resistance in human skeletal muscle. Ten subjects with impaired glucose tolerance (IGT) and 10 control subjects were recruited. We performed biopsies on the vastus lateralis muscle and used immunoblotting to determine levels of inflammatory cytokines, Toll-like receptor (TLR) gene expression, and insulin signaling. We also used a human myotube culture system to examine the mechanisms underlying TLR-4 gene expression. To identify inflammatory cytokines associated with insulin resistance, we measured the levels of IL-6, TNF-α, TLR-2, and TLR-4 in skeletal muscle from non-obese patients with IGT and control subjects. Levels of IL-6, TNF-α, and TLR-4, but not TLR-2, were significantly increased in the IGT group. Insulin resistance decreased significantly in HSMMs following long-term IL-6 treatment. TLR-4 gene expression was significantly increased in human skeletal muscle myoblasts (HSMMs) treated with IL-6. To determine the main signaling pathway for IL-6-induced TLR-4 gene expression, we examined several signaling factors associated with IL-6 signaling pathways. We found that the active form of "signal transducer and activator of transcription 3" (STAT3) was increased. "Stattic" (a STAT3 inhibitor) markedly inhibited TLR-4 gene expression. IL-6 induction of TLR-4 gene expression via STAT3 is one of the main mechanisms underlying insulin resistance in human skeletal muscle.
MeSH terms
AdultCells, CulturedFemaleGene Expression/*drug effectsGlucose Clamp TechniqueGlucose Intolerance/metabolismGlucose Tolerance TestHumansInsulinInsulin Resistance/*physiologyInterleukin-6/analysis/*pharmacologyMaleMicroscopy, Electron, TransmissionMuscle Fibers, Skeletal/metabolismMuscle, Skeletal/chemistry/drug effects/*metabolismMyoblasts/metabolismRNA, Messenger/analysisRNA, Small Interfering/pharmacologySTAT3 Transcription Factor/genetics/*physiologySignal TransductionToll-Like Receptor 2/analysisToll-Like Receptor 4/analysis/*geneticsTumor Necrosis Factor-alpha/analysis
DOI
10.1007/s00592-011-0259-z
PMID
21293887
Appears in Collections:
Journal Papers > Research Organization > Chronic Inflammatory Disease Research Center
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Journal Papers > School of Medicine / Graduate School of Medicine > Endocrinology & Metabolism
AJOU Authors
최, 성이한, 승진김, 혜진김, 대중강, 엽이, 관우
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