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Transforming growth factor (TGF)-beta1-induced human endometrial stromal cell decidualization through extracellular signal-regulated kinase and Smad activation in vitro: peroxisome proliferator-activated receptor gamma acts as a negative regulator of TGF-beta1.

Authors
Chang, HJ; Lee, JH; Hwang, KJ; Kim, MR; Chang, KH; Park, DW; Min, CK
Citation
Fertility and sterility, 90(4 Suppl.):1357-1365, 2008
Journal Title
Fertility and sterility
ISSN
0015-02821556-5653
Abstract
OBJECTIVE: To investigate the effect of transforming growth factor (TGF)-beta1 on the extracellular signal-regulated kinase (ERK) and Smad pathway and the role of peroxisome proliferator-activated receptor (PPAR)-gamma in cultured human endometrial stromal cells.



DESIGN: Experimental study.



SETTING: Infertility center of a tertiary university hospital. MATERIAL(S): Human endometrial tissues obtained by hysterectomy from patients with conditions other than endometrial diseases.



INTERVENTION(S): Endometrial stromal cells were cultured under normal laboratory conditions. TGF-beta1, rosiglitazone (PPARgamma agonist), and PD98059 (ERK inhibitor) were added to endometrial stromal cell culture according to experimental purposes.



MAIN OUTCOME MEASURE(S): Cell count, PRL expression, Smad and ERK phosphorylation, cyclooxygenase (COX)-2 expression, and prostaglandin E(2) (PGE(2)) release.



RESULT(S): TGF-beta1 inhibited cellular proliferation and induced the expressions of COX-2, PGE(2), and PRL of cultured human endometrial stromal cells. These effects may be mediated by Smad and ERK phosphorylation. Treatment with rosiglitazone, a PPARgamma agonist, reversed the TGF-beta1 effect by antagonizing the activation of ERK and Smad that was induced by TGF-beta1.



CONCLUSION(S): PPARgamma plays a negative role by directly acting on Smad and ERK phosphorylation in human endometrial cell decidualization that is induced by TGF-beta1 in vitro.
MeSH terms
AdultCells, CulturedDown-RegulationEndometrium/metabolism*Extracellular Signal-Regulated MAP Kinases/metabolism*FemaleHumansPPAR gamma/metabolism*Signal Transduction*Smad Proteins/metabolism*Stromal Cells/metabolism*Transforming Growth Factor beta1/metabolism*
DOI
10.1016/j.fertnstert.2007.09.010
PMID
18082740
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Obstetrics & Gynecology
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Science
AJOU Authors
황, 경주김, 미란장, 기홍
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