33 333

Cited 35 times in

Involvement of Ca2+-mediated apoptotic signals in palmitate-induced MIN6N8a beta cell death.

Authors
Choi, SE; Kim, HE; Shin, HC; Jang, HJ; Lee, KW; Kim, Y; Kang, SS; Chun, J; Kangv, Y
Citation
Molecular and cellular endocrinology, 272(1-2):50-62, 2007
Journal Title
Molecular and cellular endocrinology
ISSN
0303-72071872-8057
Abstract
The extracellular Ca(2+) chelator EGTA and L-type Ca(2+) channel blockers, such as, nifedipine and nimodipine were found to have a protective effect on palmitate-induced MIN6N8a beta cell apoptosis, whereas the Ca(2+) channel opener, Bay K8644, enhanced the apoptotic process. Moreover, the phospho-form of Bad, in conjunction with phospho-Akt, was reduced in response to palmitate and the palmitate-induced dephosphorylations of Akt and Bad were dependent on Ca(2+) influx. The transient expression of catalytically active Akt prevented MIN6N8a cells from palmitate-induced apoptosis. Deltamethrin, an inhibitor of Ca(2+)-activated phosphatase, delayed Akt and Bad dephosphorylations, and then protected MIN6N8a cells from palmitate-induced apoptosis. On the other hand, palmitate was found to induce CHOP, an apoptotic transcription factor in response to ER stress, and this induction was enhanced by Ca(2+) influx. Our studies suggested that Ca(2+) influx and subsequent Ca(2+)-mediated apoptotic signals are involved in palmitate-induced beta cell death.
MeSH terms
AnimalsApoptosis/drug effects*Calcineurin/physiologyCalcium/antagonists & inhibitorsCalcium/physiology*Cell Death/drug effectsCell LineChelating Agents/pharmacologyEndoplasmic Reticulum/drug effectsInsulin-Secreting Cells/drug effects*Insulin-Secreting Cells/physiologyMaleMiceOncogene Protein v-akt/metabolismPalmitic Acid/pharmacology*Protective Agents/pharmacologyRatsRats, Sprague-DawleySignal Transduction/drug effectsbcl-Associated Death Protein/metabolism
DOI
10.1016/j.mce.2007.04.004
PMID
17507155
Appears in Collections:
Journal Papers > Research Organization > Chronic Inflammatory Disease Research Center
Journal Papers > School of Medicine / Graduate School of Medicine > Endocrinology & Metabolism
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
최, 성이이, 관우강, 엽
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse