4 277

Cited 0 times in

KR-31466, a benzopyranylindol analog, attenuates hypoxic injury through mitochondrial K(ATP) channel and protein kinase C activation in heart-derived H9c2 cells.

Jung, YS; Kim, MY; Kim, MH; Lee, S; Yi, KY; Yoo, SE; Lee, SH; Baik, EJ; Moon, CH; Cho, JP
Journal of pharmacological sciences, 92(1):13-18, 2003
Journal Title
Journal of pharmacological sciences
In the present study, we investigated whether a novel benzopyranylindol analogue, KR-31466 (KR466) (1-[(2S,3R,4S)-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-6-nitro-2H-1-benzopyran-4-yl]-1H-indole-2-carboxylic acid ethyl ester) can attenuate hypoxic injury in heart-derived H9c2 cells and, if so, whether the protective effect of KR466 is mediated through mitochondrial ATP-sensitive potassium (mtK(ATP)) opening. The treatment of H9c2 cells with KR466 (3 - 30 microM) significantly reduced hypoxia-induced cell death in a concentration-dependent manner, as shown by lactate dehydrogenase release and propidium iodide-uptake. In addition, KR466 (10 microM) significantly reduced the increase in hypoxia-induced TUNEL-positive cells, suggesting its anti-apoptotic potential in H9c2 cells. The protective effects of KR466 were abolished by 5-hydroxydecanoate, a specific blocker of the mtK(ATP) channel, suggesting the involvement of the mtK(ATP) channel in the protective effect of KR466. A specific inhibitor of protein kinase C (PKC), chelerythrine (3 microM), significantly attenuated the protective effect of KR466 against hypoxia-induced cardiac cell death. In conclusion, our results suggest that KR466 can protect H9c2 cells from hypoxia-induced death through mtK(ATP) channel opening and PKC activation.
MeSH terms
AnimalsBenzopyrans/pharmacology*Cell Hypoxia/drug effectsCell LineEnzyme ActivationIn Situ Nick-End LabelingIndicators and ReagentsIndoles/pharmacology*L-Lactate Dehydrogenase/metabolismMembrane Proteins/metabolism*Myocardium/cytologyMyocardium/metabolism*Potassium ChannelsPropidium/metabolismProtective Agents/pharmacology*Protein Kinase C/metabolism*Rats
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Emergency Medicine
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
정, 이숙정, 윤석이, 수환백, 은주문, 창현조, 준필
Full Text Link
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.