41 188

Cited 0 times in

Mobility shift assay of calcium-binding proteins of mouse epididymal spermatozoa.

Authors
Gye, MC; Park, S; Kim, YS; Ahn, HS
Citation
Andrologia, 33(4):193-198, 2001
Journal Title
Andrologia
ISSN
0303-45691439-0272
Abstract
The calcium-binding proteins (CBPs) of mouse epididymal spermatozoa were analysed by mobility changes in the presence of added Ca2+ in two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The expression patterns of relatively high molecular weight CBPs (Mr > 20 kDa) were different between caput and cauda epididymal spermatozoa. There was a constitutive expression of low molecular weight CBPs (Mr < 20 kDa) regardless of the epididymal region. Most of the CBPs disappeared after the acrosome reaction (AR) induced by Ca2+ ionophore A23187, suggesting that they originated from the acrosome and/or the plasma membrane overlaying the acrosome. Taken together, it can be suggested that changes in CBPs of spermatozoa are important features of sperm maturation during epididymal transit, and that they may be related to the fertilizing ability of mouse epididymal spermatozoa.
MeSH terms
Acrosome Reaction/drug effectsAcrosome Reaction/physiologyAnimalsCalcimycin/pharmacologyCalcium/pharmacologyCalcium-Binding Proteins/chemistry*Edetic Acid/pharmacologyElectrophoresis, Polyacrylamide GelEpididymis/cytology*FertilizationIonophores/pharmacologyMaleMiceMice, Inbred ICRMolecular WeightSpermatozoa/chemistry*Spermatozoa/physiologyZinc/pharmacology
PMID
11472330
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
Journal Papers > School of Medicine / Graduate School of Medicine > Urology
AJOU Authors
김, 영수안, 현수
Full Text Link
Files in This Item:
Full-Text Not Available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse