Effects of Intravesical Heparin Treatment on the Permeability of Bladder Mucosa in Allergic Cystitis in Rat
Allergy성 방광염을 유발시킨 백서에서 Heparin의 방광내주입이 방광점막투과성에 미치는 영향
신, 현철; 박, 동춘; 김, 영수
Taehan Pinyogikwa Hakhoe chi, 39(2):116-120, 1998
Taehan Pinyogikwa Hakhoe chi; The Korean journal of urology; 대한비뇨기과학회지
Purpose: The relative impermeability of the bladder mucosa is due to the glycosaminoglycan layer covering the urothelium and the tight junction of the
urothelium. Recently, one of the most popular theories of interstitial cystitis is the penetration of urinary irritants into the suburothelial tissue due to an increased
permeability of the urothelium. This study was performed to evaluate the effect of the
intravesical heparin treatment on the permeability of bladder mucosa in allergic cystitis.
Materials and Methods: Rats were sensitized by intraperitoneal injection of ovalbumin(10mg/m1/kg) given on days 1, 3 and 5. The experiments were performed 4 weeks afrer the last injection. Controls were run simultaneously with the sensitized animals. Sensitized rats were challenged with intravesical ovalbumin(10mg/m1, 1ml) and
control rats received 1 ml saline Intravesically. Sensitized-antigen challenged group was divided into two subgroups; rats treated with intravesical hepanin(5mg/ml in 0.9% NaCl) or those treated with 1 ml saline intravesically Immediately following the intravesical
heparin(or saline) treatment, 1ml of 14C-urea was placed into the bladder for two hours. We examined the peripheral blood concentration of 14C-urea at periods up to 120 minutes.
Results: There was no 14C-urea present in the blood in control group.
There was a progressive increase in the blood level of 14C-urea with time in the sensitized-antigen challenge group. Compared with intravesical saline treatment
group, there was less progressive increase in the blood level of 14C-urea with time in the intravesical heparin treatment group. W8 also measured radioactivity of 14C-urea in the bladder tissues and found significantly lower level of 14C-urea in the bladder tissues from intravesical heparin treatment group than intravesical saline treatment group.
Conclusions: This study indicates that immunologically induced cystitis increases
bladder mucosal permeability in rats and intravesical heparin treatment decrease the
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