12 241

Cited 30 times in

Involvement of the TLR4 (Toll-like receptor4) signaling pathway in palmitate-induced INS-1 beta cell death.

Authors
Lee, SM; Choi, SE; Lee, JH; Lee, JJ; Jung, IR; Lee, SJ; Lee, KW; Kang, Y
Citation
Molecular and cellular biochemistry, 354(1-2):207-217, 2011
Journal Title
Molecular and cellular biochemistry
ISSN
0300-81771573-4919
Abstract
Fatty acid-induced cytotoxicity is believed to recapitulate lipotoxicity seen in obese type-2 diabetes, and, thus, contribute to beta cell loss in the disease. These studies were initiated to determine whether the Toll-like receptor (TLR) signaling pathway was involved in palmitate-induced beta cell death. Treatment of INS-1 beta cells with palmitate enhanced interaction between TLR and myeloid differentiation factor88 (MyD88). Concomitant with TLR/MyD88 interaction, the level of phospho-C-Jun N-terminal kinase (phospho-JNK) showed an increase; however, the level of inhibitory factor kappa B alpha (IκBα) showed a decrease. Gene knockdown of TLR4 prevented palmitate-induced INS-1 cell death, while knockdown of TLR2 did not. In addition, gene knockdown of TLR4 prevented palmitate-induced increase of phospho-JNK and decrease of IκBα. JNK inhibitor SP60125 significantly protected against palmitate-induced INS-1 cell death, while IκB kinase (IKK) inhibitor acetylsalicylate did not. These data suggest involvement of JNK activation through the TLR4 signaling pathway in palmitate-induced INS-1 beta cell death.
MeSH terms
Animals*ApoptosisCaspase 3/metabolismCell Line, TumorDNA FragmentationEnzyme ActivationI-kappa B Proteins/metabolismImmunoprecipitationInsulin-Secreting Cells/*drug effects/metabolismJNK Mitogen-Activated Protein Kinases/metabolismLipopeptides/pharmacologyMyeloid Differentiation Factor 88/metabolismPalmitatesPhosphoproteins/metabolismPoly(ADP-ribose) Polymerases/metabolismProtein BindingRNA InterferenceRats*Signal TransductionToll-Like Receptor 2/agonists/genetics/metabolismToll-Like Receptor 4/genetics/*metabolism
DOI
10.1007/s11010-011-0820-7
PMID
21503675
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Endocrinology & Metabolism
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
이, 관우강, 엽
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse